Question: calculating fold change
0
gravatar for sh
5 months ago by
sh0
sh0 wrote:

Hi everyone i am confused with a very simple problem!! i have a list include 1074 diffrentially expression genes How to determine fold change this genes?

fold change • 289 views
ADD COMMENTlink modified 5 months ago by Barry Digby630 • written 5 months ago by sh0

How did you get this list of differentially expressed genes?

ADD REPLYlink written 5 months ago by ashish570

i performed meta-analysis in 11 microarray datasetes which was contained 307 tumor samples and 197 control samples

ADD REPLYlink written 5 months ago by sh0

then your FC is the average normalized value divided by the average normalized value in controls. If you did this in R, your table should contain log2(FC), otherwise please explain the method of how this was analyzed

ADD REPLYlink written 5 months ago by JC12k
0
gravatar for Barry Digby
5 months ago by
Barry Digby630
National University of Ireland, Galway
Barry Digby630 wrote:

Take a look at this chapter, it explains very clearly how to calculate fold change

https://www.mrc-lmb.cam.ac.uk/genomes/madanm/microarray/chapter-final.pdf

ADD COMMENTlink written 5 months ago by Barry Digby630
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