calculating fold change
1
0
Entering edit mode
14 months ago
sh ▴ 10

Hi everyone i am confused with a very simple problem!! i have a list include 1074 diffrentially expression genes How to determine fold change this genes?

fold change • 730 views
ADD COMMENT
0
Entering edit mode

How did you get this list of differentially expressed genes?

ADD REPLY
0
Entering edit mode

i performed meta-analysis in 11 microarray datasetes which was contained 307 tumor samples and 197 control samples

ADD REPLY
0
Entering edit mode

then your FC is the average normalized value divided by the average normalized value in controls. If you did this in R, your table should contain log2(FC), otherwise please explain the method of how this was analyzed

ADD REPLY
0
Entering edit mode
14 months ago
Barry Digby ▴ 780

Take a look at this chapter, it explains very clearly how to calculate fold change

https://www.mrc-lmb.cam.ac.uk/genomes/madanm/microarray/chapter-final.pdf

ADD COMMENT

Login before adding your answer.

Traffic: 2099 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6