calculating fold change

Question

[sh]

Hi everyone i am confused with a very simple problem!! i have a list include 1074 diffrentially expression genes How to determine fold change this genes?

[ashish]

How did you get this list of differentially expressed genes?

[sh]

i performed meta-analysis in 11 microarray datasetes which was contained 307 tumor samples and 197 control samples

[JC]

then your FC is the average normalized value divided by the average normalized value in controls. If you did this in R, your table should contain log2(FC), otherwise please explain the method of how this was analyzed

Answer 1

[Barry Digby]

Take a look at this chapter, it explains very clearly how to calculate fold change

https://www.mrc-lmb.cam.ac.uk/genomes/madanm/microarray/chapter-final.pdf