I have 13 clusters from human single-cell RNA-seq analysis and I also have mouse RNA-seq data. I would like to compare the 13 clusters from single-cell data (Human) with mouse bulk RNA-Seq. I am thinking the following
Step1) First, convert the mouse genes to human genes Step2) Overlap these converted genes with the human genes from clusters Step3)Take the overlapped genes and their log2 fold changes from both human (single-cell RNA-seq) and mouse (bulk RNA-seq) Step4)Compute the Pearson correlations between the values (obtained from step3).
Is this the correct way to go about it.
Please guide me if this correct way to go about it. It would be great if some point out to literature as an example.
Thank you, Sai