Hey everyone. I have a bam file which I converted to sam (via samtools) and then used Picard's SamToFastq script and split the bam into two fastq files. I am now trying to run the de novo assembler Trinity on these files. I know they are strand specific, but I do not know their orientation. Are they forward-reverse orientation or reverse-forward? Which strand is the left and which is the right? Does anyone know how to determine this?