Ngsrich Output Explanation
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9.0 years ago
mark.dunning ▴ 220

I am trying to use the NGSrich tool to compute and visualise enrichment of target regions in an exome-sequencing experiment.

http://sourceforge.net/projects/ngsrich/

The tool seems to produce some useful output to hand to my collaborators, but I am not clear on the metrics reported in the HTML file. Does anyone have any detailed explanation of these metrics? Specifically,

The #target regions metric seems to differ if I gave the same target regions file, but different bam files

Do the Coverage metrics refer to On-target reads only? And on a per-base or per-Amplicon basis? What exactly are the percentage reported?

Regards,

Mark

target enrichment • 2.9k views
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is there an example of the output somewhere?

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Have you tried asking the authors? The package does not seem to have documentation that includes that level of detail.

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9.0 years ago
mark.dunning ▴ 220

Here is an example of the stats produced on the HTML page. I feel that some explanation would be useful

enter image description here

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i speculate the covered metrics are almost certainly the fraction of the regions of interest covered at varying depths. "Covered 1x" makes no sense otherwise (how could on-target+off-target be anything but 100%)

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