Hi every one,
I am going to predict the target genes of miRNAs using psRNATarget. I am using a genome assembly consisting of long contigs as the scaffold and there isn't any GFF3 file for my plant species. The problem is that when the contigs, identified by psRNATarget, are subjected to BLASTX, a lot of genes are found. Could you please guide me on how I can identify the right target genes of my miRNAs? I am so thankful to let me know if you have any recommendations.
Best regards, Mina