Entering edit mode
3.6 years ago
mingcongxu64
•
0
I want to know how to use the data from database "mouse organogenesis cell atlas" (MOCA) to analyze the characteristics of cell clusters(t-SNE visualization and marker-gene identification).
I guess you refer to https://oncoscape.v3.sttrcancer.org/atlas.gs.washington.edu.mouse.rna/downloads, right?
There are several downloads available, including a count matrix as starting point. Please read standard literature such as the Seurat vignette and the Bioconductor single-cell workflow (OSCA) to get a background. These two resources are for R users. If you prefer Python then ScanPy should get you started. You are very welcome to ask specific questions but hands-on step-by-step guidance is generally not what people provide here, therefore please edit your question, try to be precise.