Tophat Installation
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11.9 years ago
KS ▴ 380

hello

I am trying to install TopHat on my windows 7 system and I am trying to follow the steps given http://tophat.cbcb.umd.edu/tutorial.html and I am lost in this process. Could any one guide me in stalling TopHat on windows system.

Is TopHat fusion algorithm installed on Galaxy Server?

rna-seq tophat sequencing • 5.7k views
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A bit more information would be helpful. How are you trying to install it? Via Cygwin?

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I'm trying via Cygwin, but I never did it, could you tell me where can I find more information about install it?

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While not what you are looking for, you should know that Tophat is no longer the "advisable" tool for RNA-seq analysis. The software is deprecated/ in low maintenance and should be replaced by HISAT2, StringTie and ballgown. See this paper: Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown. (If you can't get access to that publication, let me know and I'll -cough- help you.) There are also alternatives such as STAR followed by htseqcounts or featureCounts and DESeq2 or edgeR...

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11.9 years ago
JC 13k

Tophat runs in Linux or Mac OS X systems, not Windows.

Tophat 2 now includes fusion detection. Just wait until the Galaxy servers update their version (if you can wait).

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