Entering edit mode
3.6 years ago
benjytan1488
•
0
Hi,
Can anyone please point me to the right direction on how to process my metabolome data? What I received was a Excel spreadsheet as shown below.
I tried using MetaboAnalyst and was able to at least analyze the statistical parts. But I was unable to perform enrichment / pathway analysis.
Viewing my data, I noticed some metabolites have duplicates with different TMS. For example, I have Glutamine-3TMS and Glutamine-4TMS and I have Glucuronic acid-meto-5TMS(1) and Glucuronic acid-meto-5TMS(2). What do these different TMS or duplicate means?
I'm not really familiar with metabolomics, so could someone please share some resources for my understanding?
Thank you very much.
Those are derivatives :
https://webbook.nist.gov/cgi/inchi?ID=R439028
https://webbook.nist.gov/cgi/inchi?ID=R620056
I see. If those are derivatives, which values should I use for the analysis? For example,
Glutamine-3TMSandGlutamine-4TMS, how can I combine those results? Or do I have to pick 1 derivative to use?Someone else will have to answer that since I don't know anything about metabolomics. I just posted those links to add some information.
Could seek advice from the core facility or company who ran the experiment. Often, these metabolites are by-products of the same pathway. In metabolomics, there is still much to be done - we still do not know the identity of ~90% of the metabolites that can be identified in blood. If you check my publications, you'll find a few metabolomics studies, so, you could follow those, too.