"C strand" and "G strand" from bisulfite converted bam files
0
0
Entering edit mode
3.6 years ago
irfanwustl ▴ 80

We know to calculate methylation value for a CpG position from the bam file we have to consider "C strand" and "G strand". I am a little confused about how to determine which read is "C strand" and which is "G strand" from the bam file? It seems a typical bisulfite aligner adds a tag to the bam file about the Bisulfite conversion strand label (like the YD tag here). I guess I have to use this tag but not sure how.

Methylation bam Bisulfite • 776 views
ADD COMMENT

Login before adding your answer.

Traffic: 1960 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6