Question: Sequence Duplication levels
0
gravatar for nageshprabhu.k
5 weeks ago by
nageshprabhu.k0 wrote:

Hello all,

I have a question regarding duplication levels shown in MultiQC report. The raw data went through HTStream for preprocessing and MultiQC was used to check. In the report I saw that when I ran multiQC on 3 different lanes of the same sample it had about 40% duplication for all three lanes (L001, L002, L003 had 40-41%) but the same sample had about 60% duplication when all 3 lanes were combined. Can anyone explain the reason for this?

Any help is appreciated! Thank you.

ADD COMMENTlink modified 5 weeks ago by genomax91k • written 5 weeks ago by nageshprabhu.k0
0
gravatar for genomax
5 weeks ago by
genomax91k
United States
genomax91k wrote:

As the amount of data increased it is possible that sequences added to the pool led to discovery of more duplicates (that were originally in separate lanes).

ADD COMMENTlink written 5 weeks ago by genomax91k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1583 users visited in the last hour