GTF/GFF3 file compatible with (GRCh38/hg38)(hg38)
0
0
Entering edit mode
3.6 years ago
kanwarjag ★ 1.2k

I am using Reference genome -Human Dec2013 (GRCh38/hg38)(hg38) and gene model Homo_sapiens.GRCh38.101.gff3.gz however I get error saying chromosome naming is not matching. I know that Ensembel uses Chr as prefix. The question is if I use Human Dec2013 (GRCh38/hg38)(hg38) as reference assembly, which gene model file of hg38 should be compatible without editing gene model gtf or gff3 file and from where I can download? Will Gencode GRCh38 GTF file be compatible with Human Dec2013 (GRCh38/hg38)(hg38)? Thanks

RNA-Seq • 1.8k views
ADD COMMENT
0
Entering edit mode

Is the reference genome from NCBI..? If yes, NCBI also provides gff.

ADD REPLY
0
Entering edit mode

I would download everything again from the same source and learn to always check chr names in a text editor before using.

I recommend ENSEMBL and GenCode for good, consistent resources. I have had better experiences there than with NCBI or UCSC efforts.

ADD REPLY

Login before adding your answer.

Traffic: 2072 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6