Hi everyone! It's my first approach to BLAST and to comparative genomics, and I would appreciate some help. I understood the theoric part of what these values rapresents (max score/%identities etc), but then, doing the pratical part, I have some problems. How can I actually understand if these values are ok or not? To be more clear, I will report some values so that you will understand better.
The query lenght is 370 aa The max score and total score is 340 The query cover is 97% The e-value is 1e-103 And the percentage identity is 47.45%
Now, I understand that is a good allignment, but I would appreciate so much if someone could explain me in a simple way for example HOW I can understand that the score I got is an High one, or just simply how you would interpret these datas. And more, how is the percent. identity related to matrice (BLOSUM 62) I used? WHY is this a good value?
I know I probably made some silly questions, but as I said, it's my first approach and I'm trying to understand the basis. Thank you so much to everyone that will help.