Question: Two different library preps
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gravatar for roberts
26 days ago by
roberts0
roberts0 wrote:

Hello I have to combine two data sets from 10x in seurat. one data set was prepared following 5' library prep and the other was prepared following 3' library prep. I am trying to follow this vignette from Seurat. The issue is that I can't find "hvg.info" anywhere in my Seurat object. I was wondering if anyone knew how to do this/ and or run CCA on a Seurat object? Thanks!

rna-seq seurat R 10x • 108 views
ADD COMMENTlink modified 26 days ago by swbarnes28.9k • written 26 days ago by roberts0
2
gravatar for swbarnes2
26 days ago by
swbarnes28.9k
United States
swbarnes28.9k wrote:

Is that tutorial on their front page of tutorials? Between that, and it being from 2018, I wonder if that bit of code is obsolete.

This standard workflow is only a few weeks old, you might give it a try.

https://satijalab.org/seurat/v3.2/integration.html

ADD COMMENTlink written 26 days ago by swbarnes28.9k
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