cluster identification in seurat

Question

[roberts]

Hello is there a program to define cell clusters or cell types in seurat or is the best option to look at gene expression maps and name the clusters yourself? Thank you!

Answer 1

[jared.andrews07]

SingleR and similar programs used a correlation-based approach to label cell types. The Orchestrating Single Cell Analysis book also has a good section on this.

You can do it manually, but that leads to a fair amount of subjectivity and is often both painstaking and time-consuming. A mix of automated tools and manual curation lead to a process that is much more convenient and likely more accurate.