validation copy number variations in whole genomes

Question

[askari.arezou94]

Hello every one I have a question about the accuracy of copy number variations found in the whole genome how can I validation copy number variations found in the laboratory? which method do you offer for me?

best regards

[brunobsouzaa]

You can easily use FISH or MLPA

[askari.arezou94]

Thank you for your response can I use from cloning for confirming validation?

[brunobsouzaa]

I believe it's possible but never used this approach! Also the qPCR, never used it to validate CNVs.

[askari.arezou94]

ook, thank you. actually, I thought coloring and qPCR are the one way to validate CNVs. do you know , why never used from qPCR and Cloning to confirm copy number variations?

[brunobsouzaa]

We have a pathology lab integrated, so every CNV is confirmed by them using FISH (for somatic). For germline CNVs we use MLPA in the lab.

[askari.arezou94]

ook. thank you for response

[askari.arezou94]

Excuseme, Can i use from "gfp Crisper" for validation copy number variations?

Answer 1

[askari.arezou94]

Can I use from qPCR to confirming copy number variations?