Question: validation copy number variations in whole genomes
0
gravatar for askari.arezou94
26 days ago by
IRAN/AHVAZ
askari.arezou9430 wrote:

Hello every one I have a question about the accuracy of copy number variations found in the whole genome how can I validation copy number variations found in the laboratory? which method do you offer for me?

best regards

sequence assembly genome • 122 views
ADD COMMENTlink modified 26 days ago • written 26 days ago by askari.arezou9430

You can easily use FISH or MLPA

ADD REPLYlink written 26 days ago by brunobsouzaa360

Thank you for your response can I use from cloning for confirming validation?

ADD REPLYlink written 26 days ago by askari.arezou9430

I believe it's possible but never used this approach! Also the qPCR, never used it to validate CNVs.

ADD REPLYlink written 26 days ago by brunobsouzaa360

ook, thank you. actually, I thought coloring and qPCR are the one way to validate CNVs. do you know , why never used from qPCR and Cloning to confirm copy number variations?

ADD REPLYlink written 26 days ago by askari.arezou9430

We have a pathology lab integrated, so every CNV is confirmed by them using FISH (for somatic). For germline CNVs we use MLPA in the lab.

ADD REPLYlink written 26 days ago by brunobsouzaa360

ook. thank you for response

ADD REPLYlink written 25 days ago by askari.arezou9430

Excuseme, Can i use from "gfp Crisper" for validation copy number variations?

ADD REPLYlink written 25 days ago by askari.arezou9430
0
gravatar for askari.arezou94
26 days ago by
IRAN/AHVAZ
askari.arezou9430 wrote:

Can I use from qPCR to confirming copy number variations?

ADD COMMENTlink written 26 days ago by askari.arezou9430
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