RNASeq data with showing other cell types/decontamination
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3.6 years ago
ccha97 ▴ 60

Hello, I have a similar question to this post here (unanswered), where I have data from RNASeq performed on B cells. However upon clustering DEGs using DESeq2, there are genes which are specifically expressed in other cell types that shouldn't be there (e.g. CD3 which is expressed only on T cells). Does anyone have any approaches or recommendations to 'uncontaminate' my data (besides having to manually check every gene)?

RNASeq RNA-Seq • 487 views
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