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3.6 years ago
askari.arezou94
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30
Hello every one I have a question about the accuracy of copy number variations found in the whole genome how can I validation copy number variations found in the laboratory? which method do you offer for me?
best regards
You can easily use FISH or MLPA
Thank you for your response can I use from cloning for confirming validation?
I believe it's possible but never used this approach! Also the qPCR, never used it to validate CNVs.
ook, thank you. actually, I thought coloring and qPCR are the one way to validate CNVs. do you know , why never used from qPCR and Cloning to confirm copy number variations?
We have a pathology lab integrated, so every CNV is confirmed by them using FISH (for somatic). For germline CNVs we use MLPA in the lab.
ook. thank you for response
Excuseme, Can i use from "gfp Crisper" for validation copy number variations?