real time pcr primer design
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21 months ago
sh ▴ 10

Hi every one i have one question it might be simple!!! i was designed real time pcr primer for my gene by using Alleleid software then i performed primerblast. the result showed there are isoforms for this gene.now i don't what am i do, Could anyone help me?

primer real time pcr • 560 views
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You can design the primers from the conserved region of different isoforms if you have no idea which isoform is expressed in your experiment.

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Sorry i have a silly question; how can i design the primers from the conserved region of isoforms? thanks alot

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Are you interested in the total gene expression or in an individual isoform?

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total gene expression

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Then simply load the NCBI gene name into PrimerBLAST and use what it outputs.

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thanks for your help

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