Entering edit mode
3.5 years ago
jun.yang2
•
0
I have an issue regarding to ChIP-seq analysis. Our combio did differential peak analysis to compare conditions A vs B. When I uploaded the bidwig files I found that the gene of interest showed opposite results. From the differential peak analysis, the binding of X was upregulated, while the bigwig files showed it was obviously down (same scaling for A and B). Some genes were consistent though. Our combio did not give me an answer. Could anybody explain what caused such discrepancies?
Thanks.
It is impossible to answer this from remote without any details. Please have a meeting with the analyst and discuss your issues.