Differentially expressed genes between Seurat objects
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6 months ago
lic4010 • 0

Hi all,

I'm trying to find differentially expressed genes between two different subsets of a Seurat object. Is there a straightforward way to do this using FindMarkers() or FindVariableFeatures()? The subsets are pre- and post-treatment and my goal is to find and remove the genes that are associated with the treatment.

thanks!!

RNA-Seq Seurat Differential gene expression • 557 views
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Do you have biological replicates? I am personally a fan of pseudbulk DE for single-cell data. In my hands this denoises the issues related to sparse data quite a lot when it comes to DE testing.

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Entering edit mode
6 months ago

Keep the samples in the same Seurat object, use a model-based test, and include the condition as a latent variable in FindMarkers, i.e. test.use = "LR", latent.vars = "treatment".

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I just want to add that you should use the counts from the RNA assay instead of other assays like SCT or integrated.

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