Question: sRNA-seq. Adapter-primer contamination
0
gravatar for joaquinj12
6 weeks ago by
joaquinj120
Portugal
joaquinj120 wrote:

Hi,

I have Illumina smallRNA-seq samples with an amount of adapters (whole sequence, maybe adapter-primer contamination) around 70% of total raw reads in the fastq file. I am removing the adapters with cutadapt from the 3', so finally the number of total reads decreases substantially. I would like to know if the adapter-primer contamination may bias the biological differences in my samples. Are the real sRNA transcripts being sequenced, or are they hidden by the adapters?

Thanks,

JoaquĆ­n

sequencing rna-seq next-gen • 108 views
ADD COMMENTlink written 6 weeks ago by joaquinj120
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1220 users visited in the last hour