Annotate .fcs files with gated population classification
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12 months ago
Benni ▴ 30


I have a .fcs file that I can process with FlowJo. Here I can set gates and name these after the population the cells in these gates represent. I would like to export that classification for each cell. The simplest solution would therefore be to add the string column 'population' to the cell .fcs data that specifies the population it belongs to. Also possible would be to add a column for each gate and mark with a boolean if the cell is inside or outside that gate. However, I have not found software that has this function to export gating annotations for each cell. Do you know software tools for that?

FACS Flow Cytometry FlowJo • 237 views

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