Question: Difference between Amlicon and Enrichment libraries
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6 weeks ago by
esimonova.me0 wrote:

I am new to NGS field. Can anyone please compare benefits and drawbacks of using enrichment and amplicon libraries? What the difference in the preparation of libraries?

next-gen • 111 views
ADD COMMENTlink modified 6 weeks ago by JC12k • written 6 weeks ago by esimonova.me0
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gravatar for JC
6 weeks ago by
JC12k
Mexico
JC12k wrote:

Enrichment means you are selecting some regions (targets) to perform the sequencing, for enrichment, there are different techniques, such as PCR amplification, hybridization methods, capture methods, etc.

Amplicon means you are selecting some regions (again, the targets) using PCR amplification, so Amplicon is an Enrichment method.

ADD COMMENTlink written 6 weeks ago by JC12k
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