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Question: Adding UMAP coordinates to metadata
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gravatar for roberts
5 days ago by
roberts • 0
roberts • 0 wrote:

Hello, I want to store my umap coordinates in my seurat objects meta.data. The umap coordinates are saved in my Object under reductions in cell.embeddings. How would I add this to the meta.data? I tried running

AD007[["coordinates"]]<--AD007@reductions$umap@cell.embeddings

I keep getting the error-- Error: Cannot add more or fewer cell meta.data information without values being named with cell names. Any suggestions? Thanks!
seurat R • 86 views
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modified 5 days ago by fracarb8 • 50 • written 5 days ago by roberts • 0
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gravatar for fracarb8
5 days ago by
fracarb8 • 50
fracarb8 • 50 wrote:

In Seurat 3, you can export the coordinates to a dataframe using Embeddings().

umapCoord <- as.data.frame(Embeddings(object = AD007[["umap"]]))

You can then add the PC columns to the meta.data as you please (cbind, sapply, paste(..,collapse=),...)

For example, if you only need to add the PCs components, without modifying them or doing other processing, you can do a simple merge

metadata_table <- AD007@meta.data
metadata_table$UMI_id <- rownames(metadata_table)
umapCoord$UMI_id <- rownames(umapCoord)
metadata_table <- merge.data.frame(metadata_table,umapCoord,by = "UMI_id")
metadata_table$UMI_id <- NULL
AD007@meta.data <- metadata_table

or if you want to be "seurat compliant"

AddMetaData(AD007, metadata=metadata_table)`

Always double check that the order of the cells between the metadata and the umap match.
ADD COMMENT • link modified 3 days ago • written 5 days ago by fracarb8 • 50

Thank you for your help. Just a follow up question how do I add PC to the meta.data (sorry I am new to coding). Thank you!

ADD REPLY • link written 4 days ago by roberts • 0

Check the edited answer

ADD REPLY • link written 3 days ago by fracarb8 • 50
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