Need Help designing a workflow
0
0
Entering edit mode
9 months ago
ZBL • 0

Hello!

I'm very new here, and was forced to shift to writing a bioinformatics dissertation because almost all the wet-labs in my country are closed. I am absolutely clueless about how this works and am still studying basic Python/R in the mean time. That being said, I need help designing a workflow for the specific reason of comparing resistance gene abundance between multiple samples of soil.

Would bowtie2 or blastn be more suitable for my needs? Would I need to assemble the data from the Illumina sequencer before alignment?

Many Thanks,

Zach

alignment sequencing resistomes ARG • 227 views
ADD COMMENT
0
Entering edit mode

Have you found any papers describing what you want to do?

ADD REPLY
0
Entering edit mode

Thank you for your response! I have been reading papers for the past few weeks and some have similar goals although really just differ in the assembly or alignment tools used respectively. I apologize if I seem unknowledgeable.

ADD REPLY

Login before adding your answer.

Traffic: 1795 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6