How to obtain organelle genome
0
0
Entering edit mode
3.5 years ago
wes ▴ 90

I have an assembled genome derived from PacBio long read data (assembled using canu). I would like to fish out both mitochondria and chloroplast contig sequence from the assembled data. Any software recommendation?

I tried to blast the assembled genome against NCBI blast but failed.

There is organelle genome in NCBI but I'm not sure how to make use of it as there is many link. Wondering how to make use of these information to achieve my objective https://ftp.ncbi.nlm.nih.gov/refseq/release/mitochondrion/

genome • 927 views
ADD COMMENT
0
Entering edit mode

if full genome is not working out for you (though a correct approach) , you could select a subset of genes/proteins that are typically only present on those plastid genomes and use those for your blast.

looking at the GC content of the contigs/Scaffolds might also give you an indication

ADD REPLY
1
Entering edit mode

Thanks. I have solved the issue by using "blat" program.

ADD REPLY
0
Entering edit mode

How did you use BLAT to pull out the organelle sequences?

ADD REPLY

Login before adding your answer.

Traffic: 2527 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6