I have this question (prob a silly question). We have a set of RNASEq data that came from BGI sequencing platform. The dataset is PE 150bp (and stranded).
I am going to use HISAT2 for alignment and wondering about the strandness setting to be used. This is the exp procedure they used:
Any thoughts on what setting I should use R/RF or F/FR . By the Image I feel the latter should be used. Just trying to make sure. Thanks in advance.