I am doing genome assembly of different bacterial strains with barcoded nanopore sequenced reads. The genome size of the bacteria is 3.8Mb. After genome assembly, I am getting 2 contigs with longest contig length of 3.6 Mb for one strain and 9 contigs with 1.5 Mb contig length for another strain. Actually, the total number of reads for the strain for which I got shortest contig length was almost double that the other strain. 1) Does another round of sequencing with nanopore improves the sequence assembly quality? 2) What could be the reason of getting shortest contig length for the genome for which I had large number of reads?