Multi-group DE scRNA-seq comps without replicates
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3.9 years ago
LacquerHed ▴ 30

Wondering if its possible to do multi-group DE comparisons of 10X/Seurat data without replicates, using perhaps just the per-cell counts alone? To compare DE genes across clusters from lets say 2 genotypes and 2 treatments.Thanks.

seurat RNA-Seq scRNA-seq DESeq2 • 886 views
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3.9 years ago
khorms ▴ 230

Yes there are definitely methods for DE comparisons implemented in the commonly used single cell packages; for example, see this discussion (link) for scanpy

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Do you know of any good tutorials for doing this in Seurat? Thanks.

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