Question: How to generate WGS Multiple sequence alignment file
gravatar for Optimist
4 weeks ago by
Optimist100 wrote:

Can you please help me tools that can be used to generate an alignment file using 16 bacterial whole genome sequences. Size of the genomes varies between 3.8 Mb to 4.2 Mb.

I need the alignment file generated by Multiple Sequence aligner as an input for gubbins tool.

Which tool can be used for achieving this purpose?

Thanks a ton

ADD COMMENTlink written 4 weeks ago by Optimist100


I am not familiar with gubbins but as stated in the github page you can use snippy . Keep in mind that your genomes must be almost identical to the reference genome: link

ADD REPLYlink written 4 weeks ago by andres.firrincieli1.0k
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