Question: how to convert FASTQ to complementary sequences
gravatar for dahun73
4 weeks ago by
dahun7310 wrote:

Hello All!

I have two questions about fastq. Is there any possibility that FASTQ sequence is complementary for original RNA sequence? If so, how to covert fastq to complementary sequences(not reverse complementary)? Is there any useful tools..?

rna-seq alignment fastq • 120 views
ADD COMMENTlink modified 26 days ago by Biostar ♦♦ 20 • written 4 weeks ago by dahun7310
gravatar for genomax
4 weeks ago by
United States
genomax92k wrote:

See: A: Ht-Seq Read Count And Strand-Specificity

Are you sure you want complementary sequence? As sequence is always represented in 5'--> 3' direction you will want the reverse complement to stay in that orientation.

ADD COMMENTlink written 4 weeks ago by genomax92k
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