Question

how to convert FASTQ to complementary sequences

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3.5 years ago

dahun73 ▴ 10

Hello All!

I have two questions about fastq. Is there any possibility that FASTQ sequence is complementary for original RNA sequence? If so, how to covert fastq to complementary sequences(not reverse complementary)? Is there any useful tools..?

RNA-Seq alignment fastq • 807 views

ADD COMMENT • link updated 3.5 years ago by Biostar 20 • written 3.5 years ago by dahun73 ▴ 10

score 1 · Answer 1 · 2020-10-29

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3.5 years ago

GenoMax 141k

See: A: Ht-Seq Read Count And Strand-Specificity

Are you sure you want complementary sequence? As sequence is always represented in 5'--> 3' direction you will want the reverse complement to stay in that orientation.

ADD COMMENT • link 3.5 years ago by GenoMax 141k