Question: how to convert FASTQ to complementary sequences
0
gravatar for dahun73
4 weeks ago by
dahun7310
dahun7310 wrote:

Hello All!

I have two questions about fastq. Is there any possibility that FASTQ sequence is complementary for original RNA sequence? If so, how to covert fastq to complementary sequences(not reverse complementary)? Is there any useful tools..?

rna-seq alignment fastq • 120 views
ADD COMMENTlink modified 26 days ago by Biostar ♦♦ 20 • written 4 weeks ago by dahun7310
1
gravatar for genomax
4 weeks ago by
genomax92k
United States
genomax92k wrote:

See: A: Ht-Seq Read Count And Strand-Specificity

Are you sure you want complementary sequence? As sequence is always represented in 5'--> 3' direction you will want the reverse complement to stay in that orientation.

ADD COMMENTlink written 4 weeks ago by genomax92k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1957 users visited in the last hour