How to know if it is missing a gene or assembly error?
0
0
Entering edit mode
2.1 years ago
DNAngel ▴ 240

Hello all,

Just wondering - after assembling my reads to a reference protein-coding sequence for a bunch of genes, I happened to notice that for a couple certain clades I just did not get any sequence from them. Is this a case of a gene being lost in the clade, or are their particular sequences too divergent to be mapped to my reference? Is there a way to tell? I wlll also mention some random other species missing the gene (no sequence obtained) but nothing as consistent as from a whole clade. This was from target exon capture.

BWA Bioinformatics Assembly • 345 views
ADD COMMENT

Login before adding your answer.

Traffic: 2012 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6