NCBI Primer designing tool not recognizing exon-exon junction?

Question

[gpreising]

I have been trying to design PCR primers that span a splice junction using the NCBI Primer design tool for a gene (NW_005179589.1). It seems like said gene has 4 exons. When I go to pick primers, I select the "Primer must span an exon-exon junction" option, but even when I have everything else on default settings, I get the error message:

"No primers were found...see explanation below: Exon/exon junction cannot be found for submitted PCR template. Try search again without exon/intron requirements."

Am I missing something here? There are several introns in the gene so I'm confused as to why I'm getting this message.

Any help would be appreciated.

[GenoMax]

Which specific gene are you trying to get primers for? The accession number (NW_005179589.1) posted is for a scafold.

[gpreising]

My apologies, I'm looking at neurotensin (nts).

Answer 1

[GenoMax]

I was able to get results using XM_005935615.1.

[gpreising]

Oops, I was trying to make primers using the scaffold accession. The mRNA accession works. Thanks!