Bismark methylation extract result interpretation
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Entering edit mode
10 months ago
tothepoint ▴ 600

I have identified DEGs and interested to know methylation effect if so during evolution. We performed WGBS sequencing and extracted gene of interest and performed bismark methyl extract to the output specific region BAM file. I have attached the output but confused why it shows CpG methylation over the whole region when the gene is expressed and confirmed. I have some understanding to methylation analysis hence need some direction if I am doing wrong or what need to done.

Also,How can we identify promoter region from for the identified genes. I will be grateful to you all for your key inputs.

enter image description here

https://ibb.co/SR4B6kH

Regards

Devender Arora

WGBS bismark methylation CpG • 371 views
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Entering edit mode
10 months ago
Ventrilocus ▴ 160

Dear Devender,

The region in your plot is of only 135 bp. No gene is that short. Make sure you have input the right genomic coordinates.

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