shotgun data analysis, how to compare one sample vs n groups?
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8 months ago
lucia • 0

Greetings to all,

I am trying to analyze some data from shotgun sequencing of human fecal samples. I have rarefied my samples in order to normalize the differents depths and I used metaphlan3. From the taxonomic table obtained I need to compare one single sample against several groups of samples (each group correspond with a group of people with certain condition) and asses if the single sample is clustered whith some group in particular or not. And also, which taxons are differentially abundant between the sample and each group.

Until now, I have performed NMDS and PCoa with Anosim to test the clusters.

My question is: wich statistic test or wich tool should I use to asses which taxons are differentially abundant between the sample and each group?

(Also I tested Lefse, but it does not accept one single sample as input to compare).

Any help would be appreciated, kind regards

LucĂ­a

statistics shotgun human gut microbiota • 252 views
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