I need know if someone has performance detection of single nucleotide variants (SNVs) using RNA-seq data. I know that the gold standard method is whole genome or exome sequencing (WGS or WES), but I need to implement a method less expensive for the determination of SNVs. I found a scientific paper written by Chepelev et al. 2009 which is entitled "Detection of single nucleotide variations in expressed exons of the human genome (https://pubmed.ncbi.nlm.nih.gov/19528076/). You know it protocol?, what are your appreciations of detection of SNVs using RNA-seq data?, what would be the weak points of this type of analysis?
I will appreciate any comments or advice