I am interested in human validation of a set of genes identified as differentially expressed between 2 groups in mice and for that, I am using some control samples from a publication. I've read that to remove batch effects with DESeq2, introducing the batch in the design of the experiment as design = ~ batch + condition is recommended. However, in my case, the 2 designs are going to be exactly the same. What can I do? Do you suggest using sva (surrogate variable analysis)? Many thanks!