I have RNA-seq data coming from peripheral blood leukocytes. I have FASTQ file and I was asked to obtain unspliced tRNAs from that file. I am a beginner in RNA-Seq analysis and I do not know how I could extract unspliced tRNAs from FASTQ file. I tried to find a workflow or tool, but I could not detect any. I would like to ask:
- Is it possible to obtain unspliced tRNAs from a FASTQ file?
- If it is possible, could you suggest me a way/tool/workflow for such task?
Thanks in advance.