Entering edit mode
3.4 years ago
Lorenzo
▴
10
Hi to all,
I have RNA-seq data from 31 tissues of 2 different individuals. The tissues are exactly the same and I setted correctly the pipeline, I mean Salmon quantification and normalization, tximport and then DEA with DESeq2 and everything works very well. I'm wondering if make sense from a biological point of view comparing gene expression between the tissues of 2 different individuals under the same conditions (age, sex and so on). I mean is it possible to consider the tissues like replicates and the individuals like different conditions (e.g. treated vs untreated)? Does it make sense?
Thank you in advance for your time and suggestions!
