Question: Haplotype Calls From Amplicon Sequencing
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gravatar for Empyrean
6.7 years ago by
Empyrean160
Empyrean160 wrote:

Hi.. i have data for two groups.. one group has 10 diffent strains and other group has 5 different strains. 40 genes are selected and primers are designed for these genes. Sequencing is performed on these amplicons which has length of around 300bp. Now i need to identify haplotypes by mapping them to the reference genes. I have to identify haplotypes for each strain as well across the strains. The data i have is reference genes and 454 reads. How to proceed please suggest me.

Tried to use some softwares like Haploview, hapcut, etc..

haplotype 454 • 1.6k views
ADD COMMENTlink written 6.7 years ago by Empyrean160
0
gravatar for Empyrean
6.6 years ago by
Empyrean160
Empyrean160 wrote:

Any help on this issue.. i am struck here

ADD COMMENTlink written 6.6 years ago by Empyrean160
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