RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites
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6 months ago
ferdecha • 0

Greetings!

I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA transcripts isoforms and processing sites.

Conventional RNA-Seq experiments are not designed for the analysis of rRNA expression and generally include a RNA depletion step, which reduces rRNA signal. Notice rRNA in bacteria accounts for ~90% of total RNA.

In the context of studying rRNA processing, would the RNA-Seq approach be appropriate? If so, what rRNA treatment would you recommend to study rRNA expression levels and processing?"

Thank you in advance.

RNA-Seq rRNA ribosomal RNA processing splicing • 269 views
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Hi Ferdecha,

I am also just wandering did you able to find any solution regarding RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites. I have plan for same thing.

Best wishes

Ghulam Nabi

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