How to annotate human genome?
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20 months ago

I am trying to annotate a human genome.

There's some recent papers that have assembled the human genome from long-read sequencing. I can get the assembly from NCBI. There's a provided gbff file, but it doesn't include annotations of the actual human genes.

What's the most standard way for me to annotate this human genome? I'm a little confused in terms of which software and what files I will need. I have the current human genome reference transcriptome in fasta and the protein sequences. I was looking at using "Maker" to put this all together, but maybe there's a better way. Can I simply using the transcriptome as the ETS inputs?

Any help would be great!

genome annotate • 440 views

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