I am trying to annotate a human genome.
There's some recent papers that have assembled the human genome from long-read sequencing. I can get the assembly from NCBI. There's a provided gbff file, but it doesn't include annotations of the actual human genes.
What's the most standard way for me to annotate this human genome? I'm a little confused in terms of which software and what files I will need. I have the current human genome reference transcriptome in fasta and the protein sequences. I was looking at using "Maker" to put this all together, but maybe there's a better way. Can I simply using the transcriptome as the ETS inputs?
Any help would be great!