Entering edit mode
3.3 years ago
genevievekrause
•
0
I am hoping there is a way to get the E-values for the individual alignments of hits in different frames frames. The two hits below are of vastly different quality but are clumped together under one sum score E-value "Expect(2)". I want their individual E-values. Is there and blast output format that provides this?
Score = 63.2 bits (152), Expect(2) = 8e-14, Method: Compositional matrix adjust.
Identities = 31/55 (56%), Positives = 42/55 (76%), Gaps = 2/55 (4%)
Frame = -1
Query 1 CHASNSSCAESTNDCSGHGSCYLKYGSGVEGTTGNCFACQCKQSIVR-NSDGTSK 54
C+ N +C E+TN CSGHGSCY K+GS EG+ G+C+AC+C +++VR N DGT K
Sbjct 20356489 CYNDNRTCTEATNSCSGHGSCYRKFGS-KEGSKGDCYACKCGRTVVRTNQDGT*K 20356328
Score = 30.0 bits (66), Expect(2) = 8e-14, Method: Compositional matrix adjust.
Identities = 15/27 (56%), Positives = 17/27 (63%), Gaps = 6/27 (22%)
Frame = -3
Query 54 KTVQWGGSACQK------RDISSPFFL 74
KTVQWGG ACQ R+ SSP F+
Sbjct 20356331 KTVQWGGPACQSK*KQVGRN*SSPIFM 20356251