Hi everyone I met some confusing issues in edgeR. and DEG Right now I have three samples without any replicated. I wonder to know to have any possibility to combine three samples for analysis (With the replicated sample, I used DEseq2 than before but right now I don't have any idea to analyze)
data <- read.csv("Vero.D2.normalisedReadCounts.csv") bcv <- 0.2 counts <- data y <- DGEList (counts=counts, group=1:2) et <- exactTest(y, dispersion=bcv^2)
If I can adjust upon code to achieve my goal.