I'm trying to align a fastq file obtained from a concatenation of reads basecalled with guppy high accuracy in MinIT. I'm receiveing the following error message:
[WARNING] wrong FASTA/FASTQ record. Continue anyway.[M::worker_pipeline::2.165*0.99] mapped 67504 sequences [E::sam_parse1] query name too long [W::sam_read1] Parse error at line 67541 [main_samview] truncated file. Alignment pipeline failed.
Here's the command i used:
mini_align -i Deerpox_2.fq.gz -r /home/lcc88/Desktop/References/Deerpox.fasta -p Deerpox_2
Apparently modifying the query names longer than 252 is the solution but i have no idea how to do it. Does anyone know how to do it? Or possibly another solution for this problem.