Jellyfish histo usage

Question

[kishorssf91]

I am trying to run jellyfish. I run count and have a result file like *.jf. I have implemented the *.jf file in histo command. but in *.histo result i have found nothing, a 0 byte file. here are command line:

#!/bin/sh

jellyfish count -t 20 -C -m 21 -s 1G -o CL_2578_2b.jf \
    --min-quality=30 \
    /mnt/disk2/Lab_Users/Kishor/genomes_raw/all/trimmed/CL_2578_2b_R1.fq \
    /mnt/disk2/Lab_Users/Kishor/genomes_raw/all/trimmed/CL_2578_2b_R2.fq

jellyfish histo -t 20 CL_2578_2b.jf -o CL_2578_2b.histo

I have tried many times, but each time find the histo result as 0 byte, nothing inside. i am also attaching the count result. Can someone please help me to fix the issue?

Count result-
/mnt/disk2/Lab_Users/Kishor/genomes_raw/all/trimmed/CL_2578_2b_R1.fq","/mnt/disk2/Lab_Users/Kishor/genomes_raw/all/trimmed/CL_2578_2b_R2.fq"],"counter_len":4,"exe_path":"/home/software/conda/miniconda/bin/jellyfish","format":"binary/sorted","hostname":"localhost.localdomain","key_len":42,"matrix1":{"c":42,"columns":[934653412,1058181293,673702217,523253146,567618272,845602800,511086072,525412897,727638623,570921397,1035913817,156451612,934500274,991722455,885814170,752095084,154457786,375951490,831899197,85630681,630062042,841343129,399313842,898293519,778157131,5475478,254131220,234598824,332368025,907819993,668250481,205645679,628373387,575704984,849911188,1053004640,583114684,714385741,582741700,668164036,497896910,469817571],"identity":false,"r":30},"max_reprobe":126,"pwd":"/mnt/disk2/Lab_Users/Kishor/genomes_raw/all/trimmed","reprobes":[1,1,3,6,10,15,21,28,36,45,55,66,78,91,105,120,136,153,171,190,210,231,253,276,300,325,351,378,406,435,465,496,528,561,595,630,666,703,741,780,820,861,903,946,990,1035,1081,1128,1176,1225,1275,1326,1378,1431,1485,1540,1596,1653,1711,1770,1830,1891,1953,2016,2080,2145,2211,2278,2346,2415,2485,2556,2628,2701,2775,2850,2926,3003,3081,3160,3240,3321,3403,3486,3570,3655,3741,3828,3916,4005,4095,4186,4278,4371,4465,4560,4656,4753,4851,4950,5050,5151,5253,5356,5460,5565,5671,5778,5886,5995,6105,6216,6328,6441,6555,6670,6786,6903,7021,7140,7260,7381,7503,7626,7750,7875,8001],"
size":1073741824,"time":"Sun Dec 27 17:53:51 2020","val_len":7}^@^@
^@^@^@
(END)

[Ram]

Please use the formatting bar (especially the code option) to present your post better. You can use backticks for inline code (`text` becomes text), or select a chunk of text and use the highlighted button to format it as a code block. If your code has long lines with a single command, break those lines into multiple lines with proper escape sequences so they're easier to read and still run when copy-pasted. I've done it for you this time.

[kishorssf91]

thanks