Transcripts per kilobase million
1
0
Entering edit mode
8 months ago
mrashad ▴ 60

While using GTEx portal to find out tissue specificity of certain genes, I found that median TPM of these genes = zero, I just need to ask if the median TSM of certain gene in certain human tissue = zero, this means that the gene is not found in this human tissue or maybe it is found but not expressed. If there is an easy resource to let me understand this issue, please provide me.

TPM pf certain genes in certain tissues

TPM • 318 views
ADD COMMENT
2
Entering edit mode
8 months ago

The median is the value at which half of samples have the same or lower value.

If the median TPM of a gene is zero, then that means that at least half of samples have a TPM of 0.

This means that in at least half of samples none of the reads in the sequence run mapped to that gene. It does not tell you anything about how many reads were found in the other 50% of samples.

Further, a TPM of 0 just tells you that no reads were recovered, not necessarily that the gene is not expressed. It could, for example, be expressed, but at a sufficiently low level that no reads were recovered.

ADD COMMENT

Login before adding your answer.

Traffic: 1791 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6