Entering edit mode
3.2 years ago
DareDevil
★
4.3k
One of method of filtering the mRNA
from NanoString nCounter
is:
[Mean + (2 x Standard Deviation)]
of negative controls
.
If I use this filtering method, I am getting around 500 mRNA out of 750 mRNA.
I used the same formula to filter miRNA
of NanoString nCounter
platform but I get only 70 miRNA
out of 750 miRNA
.
Is it not a good approach to filter miRNA
the same way of mRNA
filtered from NanoString nCounter
?